The thermo scientific pageruler unstained protein ladder is a mixture of 14 recombinant, highly purified, unstained proteins. I have run several sdspage with 10% resolving and 5% stacking. How to merge pdfs and combine pdf files adobe acrobat dc. Two orange reference bands at 70 kda and 25 kda and one green reference band at 10 kda highlight the bluestained protein ladder. Unstained protein marker, broad range 2212 kda neb. May 18, 2015 we checked neural protein expression using this protein ladder as a marker for proteins of various molecular weights. The pcr markers may be run on polyacrylamide gels with less loading volume. In the search, enter the 5digit article number without additional packaging, i. Prestained protein ladder, broad range 10230 kda neb. The apparent molecular weight of each protein kda has. The thermo scientific pageruler prestained protein ladder is a prestained mixture of ten recombinant proteins ranging from 10 kda to 180 kda. Merge pdf online combine pdf files for free foxit software. Two orange reference bands at 70 kda and 25 kda and one.
Read 5 answers by scientists with 2 recommendations from their colleagues to the question asked by hu chengcheng on mar 29, 2016. Thermo scientific pageruler prestained protein ladder, 10 to 180 kda mixture of 10 blue, orange and greenstained proteins 10 to 180kda used as size standards in protein electrophoresis sds. Combines pdf files, views them in a browser and downloads. Neuroglobin gene therapy prevents optic atrophy and. Prestained protein ladder, broad range 10230 kda is a mixture of highly purified recombinant proteins covalently coupled to a blue dye that resolves into 12 sharp bands when electrophoresed. The thermo scientific pageruler plus prestained protein ladders are a mixture of nine recombinant proteins ranging from 10 kda to 250 kda. Unstained versus prestained protein ladders scientist solutions.
Prestained protein marker, broad range 7175 kda neb. Page 4 benchmark prestained protein ladder limited use. Opal prestained protein standard 10180kda is a threecolor protein standard with 10 prestained proteins covering a wide range molecular weights for 10 to 180kda. Features of the benchmark prestained protein ladder. The protein ladder consists of 10 prestained proteins covering a wide range of molecular weights 10 to 180 kda. Genscript offers a wide selection of protein molecular weight standards for sdspage and western blot, including prestained, unstained, peptide, westernviewable, and other standards. Pageruler plus prestained protein ladder, 10 to 250 kda. The protein ladder is conveniently packaged and ready to use with no heating, diluting or additional. Thermo scientific pageruler prestained protein ladder, 10 to 180 kda mixture of 10 blue, orange and greenstained proteins 10 to 180kda used as size standards in protein electrophoresis sdspage and western blotting. If a researcher insists on doing ingel quantification, it must be done with an unstained ladder.
The unstained protein standard, broad range is a mixture of 12 recombinant, highly purified proteins, which resolve into clearly identifiable sharp bands from 10200 kda when analyzed by sdspage and stained with coomassie brilliant blue r250 1,2 or other commercially available, readytouse protein stains. Opal prestained protein standard 10180kda mb2090500. The pack contains biotinylated protein ladder and antibiotin, hrplinked antibody. Product information thermo scientific pageruler plus.
Proteins are covalently coupled with a blue chromophore except for two reference bands one green and one red band at 25 kda and 75 kda respectively made visible when separated on. Protein marker protein standard sdspage western blot. Pcr sizer 100 bp dna ladder the norgen pcrsizer 100 bp dna ladder is prepared to ensure quality and batchtobatch consistency. With pdf merger you can merge your multiple pdf files to a single pdf file in matter of seconds. Molecular weight markers, or ladders, are a set of standards that are used for determining the approximate size of a protein or a nucleic acid fragment run on an electrophoresis gel. Does pierce pageruler prestained protein ladder a mix of. The protein ladder is supplied in a readytouse format for direct loading onto ge.
It is prestained, multicoloured peptides makes eassy to identify the protein of interst bands. The blueaqua prestained protein ladder is a blue protein standard with 11. Color prestained protein standard, broad range 11245 kda neb. For accurate protein sizing on sdspolyacrylamide gels and western blots. Proteins are covalently coupled with a blue chromophore except for three reference bands two orange bands at 30 kda and 270 kda and one green band at 52 kda when separated. Each protein standard or ladder is supplied in a readytouse format, eliminating the need to reduce, premix, or add loading dyes. Proteins are covalently coupled with a blue chromophore except for two reference bands one green and one red band at 25 kda and 75 kda respectively when separated on sdspage tris. Unstained protein ladders are more accurate for sizing proteins, as the dyes used in prestained ladders can slightly distort the apparent size of the protein ladder proteins on the gel.
The benchmark prestained protein ladder is designed for use in sdspage using trisglycine gels for estimation of protein molecular weight, and evaluation of western transfer efficiency. Cst prestained protein marker, broad range 11190 kda. Plus prestained protein ladder allowed the estimation of apparent molecular mass of each signal. These standards contain predetermined fragment or protein sizes and concentrations. Unstained protein marker, broad range is a mixture of purified proteins with known amino acid sequences. The ladder is prepared from a plasmid containing repeats of a 100 bp dna fragment. The protein concentrations are carefully balanced for even intensity. This free online tool allows to combine multiple pdf or image files into a single pdf document.
Thermo scientific pageruler plus prestained protein ladder is a mixture of nine blue, orange, and greenstained proteins 10 to 250 kda for use as size standards in protein electrophoresis sdspage and. It is prestained and has multicoloured peptides which makes it easy to identify the band of the protein of interest. The apparent molecular weight of the prestained proteins are given in the table below. Choose from a variety of protein standa rds and ladders molecular weight markers for protein electrophoresis applications.
Color prestained protein standard, broad range 10250 kda. Thermo scientific pageruler plus prestained protein ladder is a mixture of nine 9 blue, orange and greenstained proteins 10 to 250kda for use as size standards in protein electrophoresis sdspage and western blotting. Comparison of multicolor prestained protein standards precision plus protein prestained all blue, dual color, and kaleidoscope standards offer matching migration patterns and nonshifting bands for. Unstained protein standard, broad range 10200 kda new. How to find the required certificate of analysis for your product. I havent had much success so far and someone pointed out that the reducing agents in my ladder were probably cleaving the biotin off the protein i should have used a different biotin to start with. The apparent molecular weight of each protein kda has been determined by calibration against an unstained protein ladder in each electrophoresis conditions. They are resolved to sharp bands when analyzed by sdspage trisglycine and stained with coomassie blue r250 1. The highranger 1 kb dna ladder is prepared to ensure quality and batchtobatch consistency. After 50kb marker, the rest seems to stack and not separating. Blue prestained protein standard, broad range 11250 kda. Allows approximate molecular weight determination when performing sdspage analysis. The blue prestained protein standard, broad range is a mixture of highly pure, recombinant, prestained proteins, covalently coupled with a blue chromophore, that resolves into 11 sharp bands when electrophoresed.
Thermo scientific pageruler prestained protein ladder is a mixture of 10 blue, orange, and greenstained proteins 10 to 180 kda for use as size standards in protein electrophoresis sdspage and western blotting. Three different chromophores are bound to the proteins, producing a brightly colored ladder. Prestained protein ladders can have a sligtly different mobility and mass than the same peotein ladder which is not prestained. Page 3 example the apparent molecular weights of the protein. The prestained protein ladder is a threecolor protein standard with 12 prestained proteins. Prestained protein ladders and unstained protein ladders. Color prestained protein standard, broad range is a mixture of highly purified, prestained proteins, covalently coupled with a. Biotinylated protein ladder detection pack is designed to detect the molecular weight ladders on western blots when using the horseradish peroxidase hrp based western detection system. The color prestained protein standard, broad range is a mixture of highly pure, recombinant, prestained proteins, covalently coupled with a blue chromophore, and two reference bands one orange and one green at 72 kda and 26 kda, respectively, that resolves into 11 sharp bands when electrophoresed. Compare prestained protein ladders from leading suppliers on biocompare.
The protein ladder consists of 12 prestained proteins covering a wide range of molecular weights 10 to 245 kda. However, an unstained protein ladder can only be visualized following staining with coomassie, or a similar nonspecific protein stain. Pageruler plus prestained protein ladder is ready to use. The prestained protein standards consist of colored bands for easy identification and allow you to monitor the progress of an electrophoretic run. The bluelf prestained protein ladder is a threecolor protein standard with prestained proteins covering a wide range molecular weights from 3. Comparison of multicolor prestained protein standards precision plus protein prestained all blue, dual color, and kaleidoscope standards offer matching migration patterns and nonshifting bands for every color.
The unstained protein standard, broad range is a mixture of 12 recombinant, highly purified proteins, which resolve into clearly identifiable sharp bands from 10200 kda when analyzed by sdspage and stained with a commercially available, readytouse protein stain. Our pcrsizer contains ten discrete fragments ranging from 100 bp to. Unstained protein standard, broad range 10200 kda neb. Thermo scientific pageruler plus prestained protein ladder is a mixture of nine blue, orange, and greenstained proteins 10 to 250 kda for use as size standards in protein electrophoresis sdspage and western blotting. Color prestained protein standard, broad range 11245 kda. Thermo scientific pageruler plus prestained protein ladder. There is one time, the dye front even stop early even though the current is running. Elite prestained protein ladder datasheet protein ark. The unstained protein standard, broad range is a mixture of 12 recombinant, highly purified proteins, which resolve into. The protein ladder consists of 10 prestained proteins covering. Thermo scientific pageruler prestained protein ladder, 10. The hxstable prestained protein ladder is a threecolor protein standard with 12 prestained proteins covering a wide range molecular weights from 10 to 245 kda. Our pdf merger allows you to quickly combine multiple pdf files into one single pdf document, in just a few clicks. Page 4 benchmark prestained protein ladder limited use label.
Mbt092 prestained protein ladder product name product code kit packing prestained protein ladder mbt09210ln 10 lanes 50 l prestained protein ladder mbt092100ln 100 lanes 500 l description. When one nucleotide contains adenine, what type of base is the adenine attached to on the opposite. Thermo scientific pageruler plus prestained protein ladder is a mixture of nine 9 blue, orange and greenstained proteins 10 to 250kda for use as size standards in protein electrophoresis. The prestained protein ladder 245 kda is a threecolor prestained protein standard which includes protein dye for convenient protein sample staining. The thermo scientific pageruler unstained protein ladder is a mixture of 14 recombinant, highly purified, unstained proteins 10 kda to 200 kda for use as size standards in protein electrophoresis sdspage and western blotting. Covalently coupled dyes allows easy visualization of the ladder during, and after electrophoresis. Page 3 example the apparent molecular weights of the protein bands in benchmark prestained protein ladder are shown below. Color prestained protein standard, broad range is a mixture of highly purified, prestained proteins, covalently coupled with a blue chromophore, and two reference bands one orange and one green at 80 kda and 25 kda, respectively, that resolves into 12 sharp bands when electrophoresed. Pcr markers have six bands of equal intensity of 50, 150, 300, 500, 750 and 1,000bp. The protein ladder is conveniently packaged and ready to. Product information thermo scientific pageruler prestained. Five microliters of benchmark prestained protein ladder was separated on a.
This ladder contains thirteen discrete fragments ranging from 300 bp to 10,000 bp with a higher intensity. A blue chromophore is bound to all proteins, except proteins of two reference bands of 70kda and 25kda that are colored with an orange dye and one green reference band of 10kda. Resolving and identifying protein of interest using pageruler. Why do the apparent molecular weight values for the prestained. Prestained protein marker, broad range 11190 kda is a mixture of purified proteins covalently coupled to a blue dye that resolve to a series of 11 bands between 11 and 190 kda following electrophoresis. Pageruler prestained protein ladder 10 170 kda migration patterns were determined using respective nupage precast gels. The unstained protein standard, broad range is a mixture of 12 recombinant, highly purified proteins, which resolve into clearly identifiable sharp bands from 10200 kda when analyzed by sdspage and.
Benchmark prestained protein ladder must show 10 major bands when separated by electrophoresis on a novex 420% trisglycine gel. Agarose gel analysis must show that bands between 1001500 bp are distinguishable after ethidium bromide. The elite prestained protein ladder is a threecolour protein standard with 10 prestained proteins covering a wide range of molecular weights from 6. Campus protein has best prices on protein, preworkouts, amino acids, fat burners, mass gainers, vitamins, and athlete approved supplements. The prestained protein ladder v is a threecolor prestained protein standard which includes protein dye for convenient protein sample staining. Color prestained protein standard, broad range is a mixture of highly purified, prestained proteins, covalently coupled with a blue chromophore, and two reference bands one orange and one green at. The quality of the bluelf prestained protein ladder is tested on a lottolot basis to ensure consistent product quality. They are resolved to sharp bands when analyzed by sdspage trisglycine and.
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